The hepg2 cell line is commonly used in drug metabolism and hepatoxicity studies. Hepg2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15yearold caucasian male who had a welldifferentiated hepatocellular carcinoma, which is the fifth mostcommon cancer worldwide. I have cultured hepg2 cells successfully in eagles minimum essential media which contains 1gl dglucose and 2mm lglutamine, and. Well as far as i know hepg2 are naturally quite resistant to g418 requires upwards of 0. Cells of this line contain hela marker chromosomes, and were derived via hela contamination. Our first job is to listen to and observe what our customers need, and meet those needs with quality products and services. Receipt of frozen cells and starting cell culture 1 immediately place frozen cells in liquid nitrogen freezer storage.
C3a hepg2c3a, derivative of hep g2 atcc hb8065 atcc. The cells express 3hydroxy3methylglutarylcoa reductase and hepatic triglyceride lipase activities. Most cell culture assays are performed in two dimensions while the real world of an in vivo cell is threedimensional. Im gonna start to work with hepg2 cells and i would to know what are best culture conditions for these cells. The hepatic stem cell line heparg is a well established invitro model more economical and convenient than primary human hepatocytes, with a full array of functions, responses and. Hepg2 dual cells stably express two inducible reporter constructs that enable the simultaneous study of the nf.
Functional toxicogenomic assessment of triclosan in. Pdf phenolic composition and biological activities of. This line was originally thought to be derived from an epidermoid carcinoma of the larynx, but was subsequently found, based on isoenzyme analysis, hela marker chromosomes, and dna fingerprinting, to have been established via hela cell contamination. Transfex transfection of plasmid dna into hepg2 cells. Hepg2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies. Hep, g2, hepg2 human caucasian hepatocyte carcinoma the hep g2 cell line has been isolated from a liver biopsy of a male caucasian aged 15 years, with a well differentiated hepatocellular. Our first job is to listen to and observe what our customers need, and meet those needs with quality. Interestingly, the atcc american type culture collection catalog indicates that hepg2 carries a chromosome 1 rearrangement, as reported in 1980 3, which.
Receipt of frozen cells and starting cell culture 1 immediately place frozen cells in liquid nitrogen freezer storage until ready to culture. In this chapter, freezing, thawing, and subculturing procedures for hepg2. Deficiency of mir122 expression in hepg2 cells may be related to a relative. The parental cells were stably transfected at passage 48 with a human cholesterol 7 alpha. In addition, treatment with mir302a mimics inhibited hepg2 cells and. We also working on cell culture since 3 years experienced various problems on protocol of different cell lines. Hepg2 hepatocellular carcinoma cell line was purchased from the american tissue culture collection atcc. Given the there are many formulations of dmem and different versions of serum, we would like to provide the catalog.
Atcc stands ready to support our customers needs during the coronavirus pandemic. Hepatocellular carcinoma hcc is the third prevalent cause of cancer death worldwide. Quantified forces between hepg2 hepatocarcinoma and wa07. An archive of our own, a project of the organization for transformative works. Hep g2 cell line human hepatocyte carcinoma, 85011430. High permissivity of human hepg2 hepatoma cells for influenza. This line was originally thought to be derived from an epidermoid carcinoma of the larynx, but. Culture and functional characterization of human hepatoma. Stable expression and integrated hepatitis b virus genome.
You may be able to find more information about this cell line by contacting the japanese collection of research bioresources jcrb. You may be able to find more information about this cell line by contacting the japanese collection. Hepg2, heparg cells, and cryopreserved human hepatocytes were exposed to 21 compounds, with known hepatotoxicity. Every step of the way, a wide range of cell health products. Hep g2 is an immortal cell line which was derived from the liver tissue of a 15yearold american adolescent boy of european ancestry. The parental cells were stably transfected at passage 48 with a human cholesterol 7 alphahydroxylase cyp7 minigeneluciferase construct. As cellular models for in vitro liver cancer and toxicity studies, hepg2 and hep3b are the two most frequently used liver cancer cell lines. To create xenograft models of hcc, hepg2 cell lines were inoculated into the flanks of 9 nude mice bilaterally. Hepg2 cells were grown to 7090% confluence and treated with 20 mmapap for 24 hours.
Apoptotic and inhibitory effects on cell proliferation of. Hb8065 is an epithelial, hepatocellular carcinoma cell line. The inhibition of cell proliferation serially became more. Transfection and cell culture the human hepatoblastoma cell line hepg2. Sr4 uncouples mitochondrial oxidative phosphorylation. The mice were anesthetized and the liver was exposed as mentioned above. The specific inhibition of hepg2 cells proliferation by. Thank you for your understanding, patience and flexibility as atcc does everything it can to help reduce the impact of the coronavirus pandemic to our valued customers. Can anyone tell me the difference between hepg2 and hep3b. What is the most efficient method to culture hepg2 cells. Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below c, preferably in liquid nitrogen vapor, until ready.
It has been proposed that engineering the cell culture microenvironment to create. Distinctive pharmacological differences between liver. Culture of hepg2 liver cells on three dimensional polystyrene scaffolds enhances cell structure and function during toxicological challenge. Hep g2 cell line human has been used to study the cytotoxicity of carbon monoxidereleasing molecules and organic solvents on hepg2 cells and to evaluate the antiproliferative activity of aqueous extracellular polysaccharides aeps for hepg2. Phase contrast micrographs of hepg2 cells grown in. I have used hepg2 in my study about erythropoietin but i would like to know if i might expect any.
Despite recent advances in diagnosis and treatment, hcc is frequently. Metformin affects the features of a human hepatocellular. The cells express 3hydroxy3 methylglutarylcoa reductase and hepatic triglyceride lipase activities. Hepg2 cells are nontumorigenic cells with high proliferation rates and an epitheliallike morphology that perform many differentiated hepatic functions.
The assessment of an invitro model for evaluating the role of parp. Growth medium for hepg2 dmem 10% fbs penstrep 1x procedure a. What is the optimal cell concentration for a hepg2 cell culture. Hep2 atcc ccl23 homo sapiens hela contaminant carcinoma. Hep g2 cells were cultured in the presence or absence of gm at 1,000. Cell culture hepg2, h358 human lung cancer, a2058 human melanoma cell lines were obtained from atcc and were cultured in dulbeccos. B pathway, through monitoring the activity of seap, and the interferon regulatory factor irf pathway, through assessing the activity of the secreted lucia luciferase. After manual removal of differentiated cells, the stem cells were. While we are not currently experiencing delays due to this pandemic, we expect that we could see them as the situation evolves. Functional toxicogenomic assessment of triclosan in human hepg2 cells using genomewide crisprcas9 screening pu xia, xiaowei zhang, yuwei xie, miao guan, daniel l. Characterization of primary human hepatocytes, hepg2 cells. Cell line was derived from the hepatocellular carcinoma cell line, hepg2 atcc hb8065.
Hepg2 cells were routinely maintained in t75 flasks. Cells were cultured according to the method reference manual. Description c3a is clonal derivative of hep g2 that was selected for strong contact inhibition of growth, high albumin production, high production of alpha. On the other hand, the hepg2 cell line is a typical carcinoma cell line that. Established from the tumor tissue of an 8yearold black boy species.